Publications

Periplasmic Cytophaga hutchinsonii Endoglucanases Are Required for Use of Crystalline Cellulose as the Sole Source of Carbon and Energy

Yongtao Zhu, Lanlan Han, Kathleen L. Hefferon, Nicholas R. Silvaggi, David B. Wilson, Mark J. McBride, 2016. Appl Environ Microbiol 82(15):4835-45.

 

Synthesis and evaluation of vitamin D receptor-mediated activities of cholesterol and vitamin D metabolites

Kelly A. Teske, Jonathan W. Bogart, Luis M. Sanchez, Olivia B. Yu, Joshua V. Preston, James M. Cook, Nicholas R. Silvaggi, Daniel D. Bikle, Leggy A. Arnold, 2016. Eur J Med Chem 109: 238-46.

 

Streptomyces wadayamensis MppP Is a Pyridoxal 5'-Phosphate-Dependent L-Arginine α-Deaminase, γ-Hydroxylase in the Enduracididine Biosynthetic Pathway

Lanlan Han, Alan W. Schwabacher, Graham R. Moran, Nicholas R. Silvaggi, 2015. Biochemistry 54(47): 7029-40.

 

Antitumor Activity of 3-Indolylmethanamines 31B and PS121912

Margaret L. Guthrie, Preetpal S. Sidhu, Emily K. Hill, Timothy C. Horan, Premchendar Nandhikonda, Kelly A. Teske, Nina Y. Yuan, Marina Sidorko, Revathi Kodali, James M. Cook, Lanlan Han, Nicholas R. Silvaggi, Daniel D. Bikle, Richard G. Moore, Rakesh K. Singh, Leggy A. Arnold, 2015. Anticancer Res 35(11): 6001-7.

 

Sbi00515, a Protein of Unknown Function from Streptomyces bingchenggensis, Highlights the Functional Versatility of the Acetoacetate Decarboxylase Scaffold

Lisa S. Mueller, Robert W. Hoppe, Jenna M. Ochsenwald, Robert T. Berndt, Geoffrey B. Severin, Alan W. Schwabacher, Nicholas R. Silvaggi, 2015. Biochemistry 54(25): 3978-88.

 

Bacterial Renalase: Structure and Kinetics of an Enzyme with 2- and 6-Dihydro-β-NAD(P) Oxidase Activity from Pseudomonas phaseolicola

Matthew R. Hoag, Joseph Roman, Brett A. Beaupre, Nicholas R. Silvaggi, Graham R. Moran, 2015. Biochemistry 54(24): 3791-802.

 

The Aspartate-Less Receiver (ALR) Domains: Distribution, Structure and Function

Andrew F. Maule, David P. Wright, Joshua J. Weiner, Lanlan Han, Francis C. Peterson, Brian F. Volkman, Nicholas R. Silvaggi, Andrew T. Ulijasz , 2015. PLOS Pathogens 11(4): e1004795.

 

You are Lost Without a Map: Judging the Reliability of Macromolecular X-ray Crystal Structures

Audrey L. Lamb, T. Joseph Kappock, Nicholas R. Silvaggi, 2015. Biochimica et Biophysica Acta: Proteins and Proteomics 1854(4): 258-68.

 

Identification of VDR Antagonists Among Nuclear Receptor Ligands Using Virtual Screening

Kelly Teske, Premchendar Nandhikonda, Jonathan W. Bogart, Belaynesh Feleke, Preetpal Sidhu, Nina Yuan, Joshua Preston, Robin Goy, Lanlan Han, Nicholas R. Silvaggi, Rakesh K. Singh, Daniel D. Bikle, James M. Cook, Leggy A. Arnold, 2014. Nuclear Receptor Research 1: 101076.

 

Anticancer Activity of VDR-Coregulator Inhibitor PS121912

Preetpal Sidhu, Kelly Teske, Belaynesh Feleke, Nina Yuan, Margaret L. Guthrie, Grant B. Fernstrum, Nishita D. Vyas, Lanlan Han, Joshua Preston, Jonathan W. Bogart, Nicholas R. Silvaggi, James M. Cook, Rakesh K. Singh, Daniel D. Bikle, Leggy A. Arnold, 2014. Cancer Chemotherapy and Pharmacology 74(4): 787-98.

 

Structural and Functional Characterization of MppR, an Enduracididine Biosynthetic Enzyme from Streptomyces hygroscopicus: Functional Diversity in the Acetoacetate Decarboxylase-Like Superfamily

A. Maxwell Burroughs, Robert W. Hoppe, Neal C. Goebel, Bilal H. Sayyed, Tyler J. Voegtline, Alan W. Schwabacher,  T. Mark Zabriskie, Nicholas R. Silvaggi, 2013. Biochemistry 52(26): 4492-4506.

 

Evaluation of Adamantane Hydroxamates as Botulinum Neurotoxin Inhibitors: Synthesis, Crystallography, Modeling, Kinetic and Cellular Based Studies

Peter Silhar, Nicholas R. Silvaggi, Sabine Pellett, Katerina Capkova, Eric A. Johnson, Karen N. Allen, Kim D. Janda, 2012. Bioorganic & Medicinal Chemistry 21(5): 1344-48.

 

Binding Energy and Catalysis by D-Xylose Isomerase: Kinetic, Product, and X-Ray Crystallographic Analysis of Enzyme-Catalyzed Isomerization of (R)-Glyceraldehyde

Maria M. Toteva, Nicholas R. Silvaggi, Karen N. Allen, John P. Richard, 2011. Biochemistry 50(46): 10170-81.

 

Chirality Holds the Key for Potent Inhibition of the Botulinum Neurotoxin Serotype A Protease

G. Neil Stowe, Peter Silhar, Mark S. Hixon, Nicholas R. Silvaggi, Karen N. Allen, Scott T. Moe, Alan R. Jacobson, Joseph T. Barbieri, Kim D. Janda, 2010. Organic Letters 12(4): 756-759.

 

Catalytic features of the botulinum neurotoxin A light chain revealed by high resolution structure of an inhibitory peptide complex

Nicholas R. Silvaggi, David Wilson, Saul Tzipori, Karen N. Allen, 2008. Biochemistry 47(21): 5736-45.

 

Structures of Clostridium botulinum Neurotoxin Serotype A Light Chain Complexed With Small Molecule Inhibitors Highlight Active-Site Flexibility

Nicholas R. Silvaggi, Grant E. Boldt, Mark S. Hixon, Jack P. Kennedy, Saul Tzipori, Kim D. Janda, Karen N. Allen, 2007.

Chemistry & Biology 14(5): 533-42.

 

Lanthanide-Binding Tags as Tools for Macromolecular Crystallographic Structure Determination

Nicholas R. Silvaggi, Langdon J. Martin, Mark Nitz, Barbara Imperiali, Karen N. Allen, 2007. Journal of the American Chemical Society 129(22): 7114-20.

 

Double-Lanthanide-Binding Tags: Design, Photophysical Properties, and NMR Applications

Langdon J. Martin, Martin J. Hahnke, Mark Nitz, Jens Wohnert, Nicholas R. Silvaggi, Karen N. Allen, Harald Schwalbe, Barbara Imperiali, 2007. Journal of the American Chemical Society 129(22): 7106-13.

 

The X-Ray Crystal Structures of Human α-Phosphomannomutase 1 Reveal the Structural Basis of Congenital Disorder of Glycosylation Type 1a

Nicholas R. Silvaggi, Chunchun Zhang, Zhibing Lu, Jianying Dai, Debra Dunaway-Mariano, Karen N. Allen, 2006. Journal of Biological Chemistry 281(21):14918-26.

 

Inactivation of Bacterial DD-Peptidase by β-Sultams

Antonio Llinás, Naveed Ahmed, Massimiliano Cordaro, Andrew P. Laws, Jean-Marie Frère, Michael Delmarcelle, Nicholas R. Silvaggi, Judith A. Kelly, Michael I. Page, 2005. Biochemistry 44(21): 7738-46.

 

Crystal Structures of Complexes Between the R61 DD-Peptidase and Peptidoglycan-Mimetic β-Lactams: A Non-Covalent Complex with a “Perfect Penicillin

Nicholas R. Silvaggi, Helen R. Josephine, Alexandre P. Kuzin, Rajesh Nagarajan, R.F. Pratt, Judith A. Kelly, 2005. Journal of Molecular Biology 345(3): 521-533.

 

Toward Better Antibiotics: Crystallographic Studies of a Novel Class of DD-Peptidase/β-Lactamase Inhibitors

Nicholas R. Silvaggi, Kamaljit Kaur, S. A. Adediran, Rex F. Pratt, Judith A. Kelly, 2004. Biochemistry 43(22): 7046-53.

 

Crystal Structure of Phosphonate-Inhibited D-Ala-D-Ala Peptidase Reveals a Tetrahedral Transition State Analog

Nicholas R. Silvaggi, John W. Anderson, S.R. Brinsmade, R. F. Pratt, Judith A. Kelly, 2003. Biochemistry 42(5): 1199-1208.

 

Observation of Michaelis and Product Complexes in the Reaction of Peptide Substrate with a Penicillin-Sensitive D-Ala-D-Ala Peptidase

Michael A. McDonough, John W. Anderson, Nicholas R. Silvaggi, Rex F. Pratt, James R. Knox, Judith A. Kelly, 2002.

Journal of Molecular Biology 322(1): 111-122.

 

A 1.2-Å Snapshot of the Final Step of Bacterial Cell Wall Biosynthesis

Wenlin Lee, Michael A. McDonough, Lakshmi P. Kotra, Zhi-Hong Li, Nicholas R. Silvaggi, Yoshifumi Takeda, Judith A. Kelly, Shahriar Mobashery, 2001. Proceedings of the National Academy of Sciences of the United States of America 98(4): 1427-1431.